| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Mikesell, Luke (16)
|
CLFD |
47.07 |
46.71 |
-0.36 |
| 2 |
Wesley, Sam J (15)
|
MARAYS |
47.10 |
46.92 |
-0.18 |
| 3 |
Gwiazdowski, Colin T (17)
|
MARY |
47.74 |
47.36 |
-0.38 |
| 4 |
Bell, Zachary (17)
|
NCCY |
47.53 |
47.58 |
0.05 |
| 5 |
McBeth, Conner (15)
|
NCCY |
47.52 |
47.61 |
0.09 |
| 6 |
Deiderick, Gabriel C (16)
|
MAFSC |
48.61 |
48.05 |
-0.56 |
| 7 |
Antolick, Joseph J (17)
|
MASKY |
48.75 |
48.17 |
-0.58 |
| 8 |
McCurley, Aiden (15)
|
MARY |
47.67 |
48.29 |
0.62 |
| 9 |
Anderson, David (17)
|
COR |
49.90 |
48.57 |
-1.33 |
| 10 |
Leitz, JR (17)
|
MALYM |
48.32 |
48.59 |
0.27 |
| 11 |
Rakow, Connor (17)
|
FLY |
47.98 |
48.83 |
0.85 |
| 12 |
Curran, Fran M (17)
|
MAPAY |
49.42 |
48.88 |
-0.54 |
| 13 |
McKeirnan, Conor M (16)
|
AMHLBG |
49.11 |
48.89 |
-0.22 |
| 14 |
Heckman, Brody E (15)
|
MARY |
48.71 |
48.95 |
0.24 |
| 15 |
Sulc, Liam (16)
|
SFY |
49.34 |
49.00 |
-0.34 |
| 16 |
Schreffler, Ryan (16)
|
HAZY |
48.56 |
49.09 |
0.53 |
| 17 |
White, Michael C (17)
|
MARY |
48.53 |
49.12 |
0.59 |
| 18 |
Beard, Matthew (16)
|
JSY |
49.97 |
49.43 |
-0.54 |
| 19 |
Scoboria, Luke R (17)
|
MARY |
49.90 |
49.58 |
-0.32 |
| 20 |
Mendieta, Diego (18)
|
MAPFY |
50.22 |
50.06 |
-0.16 |
| 21 |
Fitzgerald, Dylan J (15)
|
MANEYS |
50.15 |
50.21 |
0.06 |
| 22 |
Smith, Jase (17)
|
MABYST |
50.27 |
50.25 |
-0.02 |
| 23 |
Thome, Cole J (15)
|
AMINDY |
49.50 |
50.46 |
0.96 |
| 24 |
Hannon, William C (17)
|
AMGCY |
49.60 |
50.82 |
1.22 |